1. General description – Motorized upright light and fluorescence

microscopy system Zeiss AxioImager Z2. The system is equipped with:

  • fully motorized slide table and stand that allows fine control and full automation of image acquisition
    • Colibri 7 – an efficient LED light source for immunofluorescence with a wide variety of wavelengths and intensities
    • Axiocam 807 mono – fast, high-sensitivity, 7-megapixel monochrome microscope camera with large fields of view
    • AxioCam MRc (medium resolution colour) CCD camera
    • APOTOME 2: structured illumination module
    • option for automated switch of fluorescent filters
ReflectorExcitation filterBeamsplitter filterEmission filterSuitable fluorophores
Zeiss Filter set 49G 365FT 395BP 445/50DAPI, Hoechst, Alexa 350
Zeiss Filter set 09BP 450 – 490FT 510BP 515FITC, Alexa 488, Fluorescein, Rhodamine Green
Zeiss Filter set 20BP 546/12FT 560BP 575 – 640PI, Cy3, Alexa555, Rhodamine, Ethidium Bromide
Zeiss Filter set 50BP 640/30FT 660BP 690/50Alexa 647, Alexa 660, TO-PRO-3-DNA, TOTO-3-DNA
Zeiss Filter set 23DBP 485/20 + 546/12DFT 500+560DBP 515-530 + 580-630For simultaneous viewing of fluorophores, suitable for Filter set 09 и Filter set 20
Zeiss Filter set 64HEBP 587/25 (HE)FT 605 (HE)BP 647/70 (HE)Alexa 568, Alexa 594, BOPRO-3-DNA, mCherry, Nile Red, Cy 3.5
  • Motorized revolver with interchangeable objectives:
ObjectiveMagnificationNACover glassWorking distance (mm)Immersion
EC Plan-Neofluarx50.16plastic/glass18.5air
EC Plan-Neofluarx100.3plastic/glass5.2air
EC Plan-Neofluarx200.5Glass2.0air
EC Plan-Neofluarx400.75Glass0.71air
Plan-Neofluarx631.25Glass0.10oil
EC Plan Neofluarx1001.3Glass0.2oil

  1. Applications
    • Analysis of histological sections (paraffin-embedded or frozen)
    • Analysis of cell cultures
  2. Staining assays compatible with the system
    • Bright field (histochemical, immunohistochemical and in situ hybridization staining assays)
    • Fluorescent immunohistochemical and in situ hybridization stains; for appropriate fluorophores please refer to the table above and online at:

https://www.micro-shop.zeiss.com/index.php?s=15861634422d8e6&l=en&p=de&f=f (Zeiss Filter assistant) and to the manufacturer of the fluorophores you use, e.g.

https://www.thermofisher.com/de/de/home/life-science/antibodies/secondary-antibodies/fluorescent-secondary-antibodies/alexa-fluor-secondary-antibodies.html

  1. Slide size – min. 25mm x 75mm, max. 60mm x 75mm
  2. Whole slide imaging – by motorized stage in x/y/z planes, creating a mosaic pattern of image tiles, creating 3D reconstructions from image stacks
  3. Available software

  1. For contact and booking
  2. Location
    • Address:

Varna 9002

55 Marin Drinov str.