1. General description– iBright™ Imaging System (Модел #: CL750, Invitrogen)
    • Specifications:
      • 9,1 MP cooled CCD camera – high sensitivity and dynamic range to help enable the detection of subtle differences in samples
      • Smart Exposure technology – provides rapid determination of optimal exposure time
      • High Dynamic Range imaging – Smart Range HDR combines optimized short and long exposures into a single HDR image
    • Simple interface
      • 22.5 x 18.0-cm field of view – large field of view for high-throughput imaging (image up to four mini or two midi blots at a time)
      • Green LED-based transilluminator – effectively excite popular DNA dyes such as ethidium bromide and SYBR Green dyes with an alternative to UV-based transilluminators
      • Expanded imaging applications – capture and analyze visible, chemiluminescent or fluorescent stained colonies on single- or multi-well cell culture plates
      • Flexible connectivity
      • Multiple image analysis options directly using the on-instrument software, or for more in-depth analysis, use iBright Analysis Software
  2. Core imaging applications
    • Colorimetric-stained protein gels
    • Fluorescent-stained protein gels
    • Fluorescent-stained nucleic acid gels
    • Colorimetric-stained membranes
    • Chemiluminescent western blots
  3. Specialty imaging applications
    • Fluorescent colonies (ex. GFP expressing)
    • Visible colonies (ex. crystal violet stained)
ApplicationWhat kind of signal can be captured?
Protein GelsImage gels stained with colorimetric stains (e.g., Coomassie) and membranes (e.g.,. Ponceau S), Image protein gels stained with fluorescent stains/labels (e.g., SYPRO Ruby)
Nucleic Acid GelsFluorescent nucleic acid stains such as Ethidium Bromide and SYBR
Chemi BlotsAll popular HRP and AP substrates
Fluorescent BlotsPopular RGB (visible range) and near-IR fluorophores such as Alexa Fluor and Alexa Fluor Plus conjugates
UniversalCustom mode to image samples containing multiple signals, such as chemiluminescence, fluorescence, colorimetric stains, and/or visible images; image display is similar to fluorescent blot mode and allows one to assign false color to any sample.

4. Hardware

Sample drawer and stageManually operated drawer with fixed stage
Filters2 filters (490-520nm excitation, 568-617nm emission)
Illumination sourceGreen LED (470-550nm) transilluminator

5. System software

iBright Imaging Systems

Available on the instrument and as a desktop version (Windows и Mac OS)

Automated features and algorithms: Automatic zoom, automatic focus, automatic exposure (Smart exposure), automatic on-board image analysis, High dynamic range (HDR) image capture functionality (Smart Range HDR**)

Workspace: Gallery, Adjust, Analyze, Annotate

** HDR available in chemiluminescent blot mode only.

6.Compatible reagents:

ModeCompatible reagents
Chemi Blots– SuperSignal West Atto
– SuperSignal Weste Pico Plus
– SuperSignal West Dura
– SuperSignal West Femto
– Pierce ECL Plus
– Pierce ECL
– Other AP and HRP chemiluminescent substrates
Protein Gels – Coomassie stains
– Silver stains
– SYPRO Ruby
– SYPRO Orange
– SYPRO Tangerine
– SYPRO Red
– Coomassie Fluor Orange
– Deep purple stains
– Oriole fluorescent stain
Nucleic Acid Gels-Ethidium bromide
-Hoechst blue
– SYBR Green I
– SYBR Green II
– SYBR Safe
– SYBR Gold
Colony counting– GFP (using transillumination)  
– Crystal violet (using transillumination)
– Blue/white visible (β-galactosidase/X-Gal/IPTG)

! For the supply of the above mentioned consumables or equivalents, please contact the respective representative companies in Bulgaria. 

7. Detailed information about iBright™ (Model #: CL750) can be found at: https://www.thermofisher.com/order/catalog/product/A44116

User guide: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018652_iBright_CL750_UG.pdf

8. For contact and booking:

Desislava Tsoneva; email: desislava.tsoneva@mu-varna.bg

9. Location

Address: Varna 9002 55 Marin Drinov str.