1. General description– StepOnePlus™ RealTime PCR System

(Catalog number: 4376600, Applied Biosystems) with the following features:

  • 96-well platform
  • Four-color system
  • VeriFlex™ block

  1. Applications
    • Quantitative detection of nucleic acid sequences (targets) by real-time amplification analysis.
    • Qualitative detection of targets using post-PCR (endpoint) analysis.
    • Qualitative analysis of the resulting PCR product (achieved by melt curve analysis, which is performed post-PCR).
  2. Experiment types selection and data collection
CategoryPropertiesExperiment type
Real-time–  The instrument monitors the progress of the PCR as it occurs. –  Data are collected throughout the PCR process. –  Reactions are characterized by the point in time during cycling when amplification of a target is first detected.Quantitation
Endpoint–  Data are collected at the end of the PCR process. –  Reactions are characterized by the quantity of the target accumulated at the end of PCR. –  Reactions The datapoint is the normalized intensity of the reporter dye, or Rn.Genotyping
Presence/absence
  1. Selection of reagent type:

You can use the following types of reagents (chemicals) on the StepOnePlus system:

  • TaqMan® reagents
  • SYBR® Green reagents
  • Other fluorescent-based reagents
ReagentsApplication
TaqMan®Quantitative analysis, including:Standard curveRelative standard curveComparative curve (ΔΔCT, cycle of quantification)GenotypingPresence/absence
SYBR® GreenQuantitative analysis, including:Standard curveRelative standard curveComparative curve (ΔΔCT)
  1. Filters: StepOnePlus uses the following filters:
FilterDye
1FAM™ dye
SYBR® Green dye
2JOE™ dye
VIC® dye
3TAMRA™ dye
NED™ dye
4ROX™ dye
  1. VeriFlex™ blocks

The StepOnePlus system contains six independently adjustable VeriFlex™ blocks that help you optimize thermal cycle conditions. You can set a different temperature for one or more of the VeriFlex blocks, creating up to six different sample zones, or you can set the same temperature for each of the VeriFlex blocks.

  1. Supported consumables:

The StepOnePlus system allows the following options:

                    Option 1                               Option 2                            Option 3
#Consumable
AMicroAmp™ Fast Optical 96-Well Reaction Plate with Barcode
BMicroAmp™ 96-Well Tray for VeriFlex™ Blocks
CMicroAmp™ 96-Well Support Base
DMicroAmp™ Optical 8-Cap Strip
EMicroAmp™ Fast 8-Tube Strip
FMicroAmp® Fast Reaction Tube with Cap
GMicroAmp™ Optical Adhesive Film

The StepOnePlus system supports the consumables listed below. These consumables are for use with both standard and Fast reagents/protocols. IMPORTANT! Use only Fast consumables (reaction plates, tube strips, and tubes) with the StepOnePlus systems, even when performing an experiment with standard reagents. IMPORTANT! The StepOnePlus system does not allow the use of 384-well plates.

ConsumableCatalogue number
(
in Applied Biosystems)
MicroAmp™ Fast Optical 96-Well Reaction Plate with BarcodeMicroAmp™ Optical Adhesive Film4346906, 4366932 or equivalent   4360954, 4311971 or equivalent
MicroAmp™ Fast 8-Tube StripMicroAmp™ Optical 8-Cap Strip4358293 or equivalent4323032 or equivalent
MicroAmp® Fast Reaction Tube with Cap4358297 or equivalent
MicroAmp™ 96-Well Tray for VeriFlex™ BlocksMicroAmp™ 96-Well Support Base4379983 or equivalent   4379590 or equivalent
MicroAmp™ Adhesive Film ApplicatorMicroAmp™ Cap Installing Tool (Handle)4333183 or equivalent4330015 or equivalent

! For the supply of the above-mentioned consumables or equivalents, please get in touch with the respective representative companies in Bulgaria.

  1. Detailed information aboutStepOnePlus qPCR can be found at: https://www.thermofisher.com/order/catalog/product/4376600 ; a user manual can be found here: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/cms_046739.pdf
  2. For contact and booking:
  3. Location:
    • Address:

Varna 9002

55 Marin Drinov str.