
- General description– StepOnePlus™ RealTime PCR System
(Catalog number: 4376600, Applied Biosystems) with the following features:
- 96-well platform
- Four-color system
- VeriFlex™ block
- Applications
- Quantitative detection of nucleic acid sequences (targets) by real-time amplification analysis.
- Qualitative detection of targets using post-PCR (endpoint) analysis.
- Qualitative analysis of the resulting PCR product (achieved by melt curve analysis, which is performed post-PCR).
- Experiment types selection and data collection
Category | Properties | Experiment type |
Real-time | – The instrument monitors the progress of the PCR as it occurs. – Data are collected throughout the PCR process. – Reactions are characterized by the point in time during cycling when amplification of a target is first detected. | Quantitation |
Endpoint | – Data are collected at the end of the PCR process. – Reactions are characterized by the quantity of the target accumulated at the end of PCR. – Reactions The datapoint is the normalized intensity of the reporter dye, or Rn. | Genotyping |
Presence/absence |
- Selection of reagent type:
You can use the following types of reagents (chemicals) on the StepOnePlus system:
- TaqMan® reagents
- SYBR® Green reagents
- Other fluorescent-based reagents
Reagents | Application |
TaqMan® | Quantitative analysis, including:Standard curveRelative standard curveComparative curve (ΔΔCT, cycle of quantification)GenotypingPresence/absence |
SYBR® Green | Quantitative analysis, including:Standard curveRelative standard curveComparative curve (ΔΔCT) |
- Filters: StepOnePlus uses the following filters:
Filter | Dye |
1 | FAM™ dye |
SYBR® Green dye | |
2 | JOE™ dye |
VIC® dye | |
3 | TAMRA™ dye |
NED™ dye | |
4 | ROX™ dye |
- VeriFlex™ blocks
The StepOnePlus system contains six independently adjustable VeriFlex™ blocks that help you optimize thermal cycle conditions. You can set a different temperature for one or more of the VeriFlex blocks, creating up to six different sample zones, or you can set the same temperature for each of the VeriFlex blocks.
- Supported consumables:
The StepOnePlus system allows the following options:
Option 1 Option 2 Option 3 |

# | Consumable |
A | MicroAmp™ Fast Optical 96-Well Reaction Plate with Barcode |
B | MicroAmp™ 96-Well Tray for VeriFlex™ Blocks |
C | MicroAmp™ 96-Well Support Base |
D | MicroAmp™ Optical 8-Cap Strip |
E | MicroAmp™ Fast 8-Tube Strip |
F | MicroAmp® Fast Reaction Tube with Cap |
G | MicroAmp™ Optical Adhesive Film |
The StepOnePlus system supports the consumables listed below. These consumables are for use with both standard and Fast reagents/protocols. IMPORTANT! Use only Fast consumables (reaction plates, tube strips, and tubes) with the StepOnePlus systems, even when performing an experiment with standard reagents. IMPORTANT! The StepOnePlus system does not allow the use of 384-well plates.
Consumable | Catalogue number (in Applied Biosystems) |
MicroAmp™ Fast Optical 96-Well Reaction Plate with BarcodeMicroAmp™ Optical Adhesive Film | 4346906, 4366932 or equivalent 4360954, 4311971 or equivalent |
MicroAmp™ Fast 8-Tube StripMicroAmp™ Optical 8-Cap Strip | 4358293 or equivalent4323032 or equivalent |
MicroAmp® Fast Reaction Tube with Cap | 4358297 or equivalent |
MicroAmp™ 96-Well Tray for VeriFlex™ BlocksMicroAmp™ 96-Well Support Base | 4379983 or equivalent 4379590 or equivalent |
MicroAmp™ Adhesive Film ApplicatorMicroAmp™ Cap Installing Tool (Handle) | 4333183 or equivalent4330015 or equivalent |
! For the supply of the above-mentioned consumables or equivalents, please get in touch with the respective representative companies in Bulgaria.
- Detailed information aboutStepOnePlus qPCR can be found at: https://www.thermofisher.com/order/catalog/product/4376600 ; a user manual can be found here: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/cms_046739.pdf
- For contact and booking:
- Desislava Tsoneva, email: desislava.tsoneva@mu-varna.bg
- Location:
- Address:
Varna 9002
55 Marin Drinov str.