- General description – Motorized upright light and fluorescence microscopy system ZeissAxioImager Z2. The system is equipped with:
- Fully motorized slide table and stand that allows fine control and full automation of image acquisition
- Colibri 7 – an efficient LED light source for immunofluorescence with a wide variety of wavelengths and intensities
- Axiocam 807 mono – fast, high-sensitivity, 7-megapixel monochrome microscope camera with large fields of view
- AxioCam MRc (medium resolution colour) CCD camera
- APOTOME 2: structured illumination module
- Option for automated switch of fluorescent filters
| Reflector | Excitation filter | Beamsplitter filter | Emission filter | Suitable fluorophores |
| Zeiss Filter set 49 | G 365 | FT 395 | BP 445/50 | DAPI, Hoechst, Alexa 350 |
| Zeiss Filter set 09 | BP 450 – 490 | FT 510 | BP 515 | FITC, Alexa 488, Fluorescein, Rhodamine Green |
| Zeiss Filter set 20 | BP 546/12 | FT 560 | BP 575 – 640 | PI, Cy3, Alexa555, Rhodamine, Ethidium Bromide |
| Zeiss Filter set 50 | BP 640/30 | FT 660 | BP 690/50 | Alexa 647, Alexa 660, TO-PRO-3-DNA, TOTO-3-DNA |
| Zeiss Filter set 23 | DBP 485/20 + 546/12 | DFT 500+560 | DBP 515-530 + 580-630 | For simultaneous viewing of fluorophores, suitable for Filter set 09 и Filter set 20 |
| Zeiss Filter set 64HE | BP 587/25 (HE) | FT 605 (HE) | BP 647/70 (HE) | Alexa 568, Alexa 594, BOPRO-3-DNA, mCherry, Nile Red, Cy 3.5 |
- Motorized revolver with interchangeable objectives:
| Objective | Magnification | NA | Cover glass | Working distance (mm) | Immersion |
| EC Plan-Neofluar | x5 | 0.16 | plastic/glass | 18.5 | air |
| EC Plan-Neofluar | x10 | 0.3 | plastic/glass | 5.2 | air |
| EC Plan-Neofluar | x20 | 0.5 | Glass | 2.0 | air |
| EC Plan-Neofluar | x40 | 0.75 | Glass | 0.71 | air |
| Plan-Neofluar | x63 | 1.25 | Glass | 0.10 | oil |
| EC Plan Neofluar | x100 | 1.3 | Glass | 0.2 | oil |
- Applications
- Analysis of histological sections (paraffin-embedded or frozen)
- Analysis of cell cultures
- Staining assays compatible with the system
- Bright field (histochemical, immunohistochemical and in situ hybridization staining assays)
- Fluorescent immunohistochemical and in situ hybridization stains; for appropriate fluorophores please refer to the table above and online at: https://www.micro-shop.zeiss.com/index.php?s=15861634422d8e6&l=en&p=de&f=f (Zeiss Filter assistant) and to the manufacturer of the fluorophores you use, e.g. https://www.thermofisher.com/de/de/home/life-science/antibodies/secondary-antibodies/fluorescent-secondary-antibodies/alexa-fluor-secondary-antibodies.html
- Slide size – min. 25mm x 75mm, max. 60mm x 75mm
- Whole slide imaging – by motorized stage in x/y/z planes, creating a mosaic pattern of image tiles, creating 3D reconstructions from image stacks
- Available software
- ZenBlue 3.7 (integrated with the microscope, image capture and processing); https://www.zeiss.com/microscopy/en/products/software/zeiss-zen.html
- StereoInvestigator (integrated with the microscope, imaging and stereological analysis of thick sections); https://www.mbfbioscience.com/products/stereo-investigator
- Neurolucida 360 (offline, analytical); https://www.mbfbioscience.com/products/neurolucida-360/
- Vesselucida 360 (offline, analytical); https://www.mbfbioscience.com/products/vesselucida-360
- BrainMaker (offline, analytical); https://www.mbfbioscience.com/products/brainmaker
- For contact and booking
- Dr. Martin Ivanov, email: martin.ivanov@mu-varna.bg
- Location
- Address: Varna 9002 55 Marin Drinov str.